HPLC Method for Analysis of 4-Aminophenol on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
4-Aminophenol (also known as para-aminophenol or Rodinal) is a popular film developing compound and building block in organic chemistry reactions. Most notably, it is a key compound used in the production of acetaminophen (also known as paracetamol or Tylenol). 4-Aminophenol can be separated, retained, and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and an sulfuric acid (H2SO4) or Perchloric acid (HClO4)buffer. This analysis method can be detected in the UV regime at 275 nm.
Methodenparameter
| Column | Primesep 100 , 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN – 10% |
| Buffer | H2SO4 – 0.2% or HClO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 200, 210, 275 nm |