HPLC Method for Analysis of 4-(2-Aminoethyl) benzenesulfonamide on Primesep 100 Column
Separation type: Liquid Chromatography Mixed-mode
4-(2-Aminoethyl) benzenesulfonamide, when combined with hypoxic conditions, can inhibit the growth and induce apoptosis of human hepatocellular carcinoma cells. This Benzenesulfonamide can be retained and analyzed on a Primesep 100 mixed-mode stationary phase column using an isocratic analytical method with a simple mobile phase of water, Acetonitrile (MeCN), and a Sulfuric acid (H2SO4) buffer. This analysis method can be detected in the low UV regime at 220 nm.
Methodenparameter
| Column | Primesep 100 , 4.6×150 mm, 5 µm, 100 Å |
|---|---|
| Mobile Phase | MeCN/H2O – 20/80% |
| Buffer | H2SO4 – 0.2% |
| Flow Rate | 1.0 mL/min |
| Detection | UV 220, 265 nm |