HPLC ELSD Method for Analysis Trifluoroacetic acid on Primesep B4 Column
Trifluoroacetic acid (TFA) is a strong organic acid with the chemical formula CF3COOH.
Trifluoroacetic acid can be retained and analyzed using an Primesep B4 mixed-mode stationary phase column. The analysis employs an isocratic method with a simple mobile phase consisting of water, acetonitrile (MeCN), and ammonium formate as a buffer. Detection is achieved using ELSD
Methodenparameter
| Column | Primesep B4, 4.6 x 150 mm, 5 µm, 100 Å, dual ended |
|---|---|
| Mobile Phase | MeCN/H2O – 70/30% |
| Buffer | Ammonium formate pH 3.0 – 60 mM |
| Flow Rate | 1.0 mL/min |
| Detection | ELSD, the nebulizer and evaporator temperatures 50°C, with a gas flow rate of 1.6 Standard Liters per Minute (SLM) |
| Injection Volume | 3 µl |