HPLC Analysis of Cefradine

HPLC-Applikation • Datum: 2015/08/06
HPLC Analysis of Cefradine Chromatogramm

Cefradine is a drug used to treat bacterial infections of the respiratory or urinary tracts and skin or soft tissue. Legacy L1 was used to retain cefradine by reverse phase mechanism.Legacy L1 uses embedded C18 groups on porous silica and is useful for many USP HPLC applications. comparisons to Phenomenex columns are available by request.

SIELC's family of Legacy columns is based on the United States Pharmacopeia's (USP) published chromatographic methods and procedures. Numerous brands have columns used in USP reference standards and methods. USP has created various designations to group together columns with similar types of packing and properties in the solid phase. SIELC's Legacy columns adhere to these strict requirements and properties, allowing you to easily replace older columns that are no longer available without needing to significantly modify your method or SOPs.

Methodenparameter

ColumnLegacy L1, 4.6×250 mm, 5 µm, 100 Å
Mobile PhaseMeOH – 15%
BufferAmFm pH 3.0- 40 mM
Flow Rate1.0 mL/min
DetectionUV, 250 nm