HPLC Analysis of Azide Ion on Obelisc N Column

Sodium azide is used in older car airbag systems and airplane escape slides. It is an acutely toxic substance. Obelisc N mixed-mode column can retain the azide ion using MS-compatible mobile phase of acetonitrile (ACN) and water with Ammonium Acetate as buffer. Can also be UV detected at 250 nm.

SIELC has developed the Obelisc™ columns, which are mixed-mode and utilize Liquid Separation Cell technology (LiSC™). These cost-effective columns are the first of their kind to be commercially available and can replace multiple HPLC columns, including reversed-phase (RP), AQ-type reversed-phase, polar-embedded group RP columns, normal-phase, cation-exchange, anion-exchange, ion-exclusion, and HILIC (Hydrophilic Interaction Liquid Chromatography) columns. By controlling just three orthogonal method parameters - buffer concentration, buffer pH, and organic modifier concentration - users can adjust the column properties with pinpoint precision to separate complex mixtures.